辣根过氧化物酶作为标记物广泛用于各种免疫化学包括酶联免疫反应、免疫印迹和免疫组化中。此外,它也用于临床检测中过氧化氢的检测。
Appearance: Reddish-brown amorphous powder, lyophilized
Source: Horseradish
Enzyme Commission Number: EC 1.11.1.7
CAS Number: 9003-99-0
Storage temperature: -20℃
Activity: ≥250 Purpurogallin U/mg solid
Unit definition: One purpurogallin unit causes the formation of one milligram of purpurogallin in 20s at pH 6.0 at 20 °C.
PROPERTIES
Stability: Stable at -20℃ for at least one year
Molecular weight ~40 kDa (SDS-PAGE)
Isoelectric point 7.2
Optimum pH: 6.5
Optimum temperature: 45℃
pH Stability 5.0~10.0(30℃,16hr)
Thermal stability < 50℃ (pH 6.0,15min)
Inhibitors Cyanide, sulfide, fluoride, azide